ZZU-iPS-Normal-001
ZZUi011-A
General#
Cell Line |
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hPSCreg Name | ZZUi011-A |
Alternative name(s) |
ZZU-iPS-Normal-001
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 13th December 2017 |
User feedback | |
Provider |
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Generator | The first affiliated hospital (ZZU) |
Owner | The first affiliated hospital (ZZU) |
Derivation country | China |
External Databases |
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BioSamples | SAMEA104620781 |
Cellosaurus | CVCL_VE21 |
General Information |
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Publications | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | male |
Ethnicity | Han |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA104620782 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
Kidney Tubules |
Source cell origin |
urineExcretion that is the output of a kidney
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Source cell type (free text) | Kidney tubules epithelial cells |
Age of donor (at collection) | 25-29 |
Collected in | 2016 |
Passage number reprogrammed | P3 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | These colonies will consist of iPSCs, which exhibit a hESC-like morphology characterized by a flatter cobblestone-like appearance with individual cells clearly demarcated from each other in the colonies. |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Vitronectin | |||||||||
Feeder cells |
No |
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Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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O2 Concentration | 95 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
TeSR™ E8™
Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SOX2 |
Yes |
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TRA 1-60 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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