ZZU-iPS-Normal-001
ZZUi011-A
General#
Cell Line |
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| hPSCreg Name | ZZUi011-A |
| Alternative name(s) |
ZZU-iPS-Normal-001
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 13th December 2017 |
| User feedback | |
Provider |
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| Generator | The first affiliated hospital (ZZU) |
| Owner | The first affiliated hospital (ZZU) |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA104620781 |
| Cellosaurus | CVCL_VE21 |
General Information |
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| Publications | |
| Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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| Sex | male |
| Ethnicity | Han |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA104620782 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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| Source cell type |
Kidney Tubules |
| Source cell origin |
urineExcretion that is the output of a kidney
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| Source cell type (free text) | Kidney tubules epithelial cells |
| Age of donor (at collection) | 25-29 |
| Collected in | 2016 |
| Passage number reprogrammed | P3 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Selection criteria for clones | These colonies will consist of iPSCs, which exhibit a hESC-like morphology characterized by a flatter cobblestone-like appearance with individual cells clearly demarcated from each other in the colonies. |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Vitronectin | |||||||||
| Feeder cells |
No |
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| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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| O2 Concentration | 95 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
TeSR™ E8™
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| TRA 1-60 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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