Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene


The KCL016 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel-Lindau tumor suppressor E3 ubiquitin protein ligase (676+3A>T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Miere C, Hewitson H, Wood V, Kadeva N, Cornwell G, Codognotto S, Stephenson E, Ilic D
Journal Stem cell research
Publication Date 2016 Jan;16(1):37-9
PubMed 27345783
PubMed Central PMC4757726
DOI 10.1016/j.scr.2015.12.003

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