Generation of four integration-free iPSC lines from related human donors


Four integration-free iPSC lines were generated by reprogramming peripheral blood mononuclear cells with episomal vectors. All four human donors (two male and two female donors) belong to one Caucasian family within three different generations with the age between 19-82 years. Additionally, all iPSC lines are approved for commercial use by donor consent. Those iPSC lines offer the opportunity to study the influence of affiliation within one family. In future, more iPSCs lines of many more family members can be created to understand the effects of relatives with different ages on the reprogramming into iPSCs and differentiation into specific cell types. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Ramme AP, Faust D, Koenig L, Marx U
Journal Stem cell research
Publication Date 2019 Dec;41:101615
PubMed 31704539
DOI 10.1016/j.scr.2019.101615

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