TISSUi003-A

HUMIMIC103, StemUse103, SU103

General

Cell Line

hPSCreg name TISSUi003-A
Cite as:
TISSUi003-A (RRID:CVCL_WU58)
Alternative name(s)
HUMIMIC103, StemUse103, SU103
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 22nd April 2022
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Provider

Generator TissUse GmbH (TISSU)
Owner TissUse GmbH (TISSU)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA5928368
Cellosaurus CVCL_WU58
Wikidata Q98133267

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 15-19
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: Molecular karyotyping by SNP array
http://

External Databases (Donor)

BioSamples SAMEA5928369

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Dr. rer. nat. Gerd Lindner Provio GmbH
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethikkomission der Ärztekammer Berlin
Approval number Eth 25/16
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethikkomission der Ärztekammer Berlin
Approval number Eth 25/16
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Age of donor (at collection) 15-19
Collected in 2016

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-5
Yes
SOX2
Yes
Pluripotency Score Novelty Score
40.00725 1.432593
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
FOXA2
Yes
Morphology
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Marker Expressed
Cardiac Troponin T (cTnT)
Yes
Myosin Heavy Chain 6 (MHC)
Yes
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
TUBB3
Yes
PAX6
Yes
Morphology
TUBB3 green, PAX6 red, DAPI blue, scale 100 µm

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
KaryoStat™ analysis of TISSUi003-A (StemUse103) revealed the sample originated from a female individual. No chromosomal aberrations were found when comparing against the reference dataset.
Passage number: P17
Karyotyping method: KaryoStat™

Other Genotyping (Cell Line)