General#

Cell Line

hPSCreg Name TUSMi004-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th October 2018
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Provider

Generator Tongji University School of Medicine (TUSM)

External Databases

BioSamples SAMEA4675908
Cellosaurus CVCL_VR56

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
obsessive-compulsive disorder
The donor is affected.
Synonyms
  • OCD

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA4675909

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Source cell origin
blood
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Yes
Methods used
Immunostaining, PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro directed differentiation
Marker Expressed
AFP
Yes
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro directed differentiation
Neuron
Ont Id: CL_0000540
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
XY,46
Karyotyping method: G-Banding

Other Genotyping (Cell Line)