Generation of a TLR7 homozygous knockout human induced pluripotent stem cell line using CRISPR/Cas9


Toll Like Receptor (TLR) family plays an important role in the activation of innate immunity against pathogens. TLR7 mediates the recognition of single-stranded RNA viruses, such as human immunodeficiency virus, hepatitis C virus, and influenza virus in endosomes. Here, we generated a TLR7 homozygous knockout human induced pluripotent cell (hiPSC) line, hiPSC-TLR7KO-A59, using the CRISPR/Cas9 genome editing method. The hiPSC-TLR7KO-A59 line maintains normal morphology, pluripotency, and differentiation capacity into three germ layers. Copyright © 2019. Published by Elsevier B.V.

Authors Han HJ, Seo HH, Han HW, Kim JH
Journal Stem cell research
Publication Date 2019 Oct;40:101520
PubMed 31445394
DOI 10.1016/j.scr.2019.101520

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