Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus


MERTK mutations are associate with rod-cone dystrophies. To enable investigations into the mechanism of this disease, we generated a cell line resource of H9 human embryonic stem cells harboring large fragment deletion mutation in a homozygous state in exon 19 of the MERTK gene. This subline expressed pluripotent stem cell markers, presented a normal karyotype, and preserved the ability to differentiate into endodermal, mesodermal, and ectodermal lineages. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Tang X, Fu J, Chen Z, Luo L, Li DW, Liu Y
Journal Stem cell research
Publication Date 2021 May;53:102310
PubMed 33812335
DOI 10.1016/j.scr.2021.102310

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