Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus
Summary
MERTK mutations are associate with rod-cone dystrophies. To enable investigations into the mechanism of this disease, we generated a cell line resource of H9 human embryonic stem cells harboring large fragment deletion mutation in a homozygous state in exon 19 of the MERTK gene. This subline expressed pluripotent stem cell markers, presented a normal karyotype, and preserved the ability to differentiate into endodermal, mesodermal, and ectodermal lineages. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Tang X, Fu J, Chen Z, Luo L, Li DW, Liu Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102310 |
| PubMed | 33812335 |
| DOI | 10.1016/j.scr.2021.102310 |