chHES-90

General#

Cell Line

hPSCreg Name NERCe002-A
Alternative name(s)
chHES-90
Cell line type Human embryonic stem cell (hESC)
Last update 20th October 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator National Engineering and Research Center of Human Stem Cell (NERC)
Owner National Engineering and Research Center of Human Stem Cell (NERC)

External Databases

BioSamples SAMEA104132871
Cellosaurus CVCL_B008

General Information

Publications
Is the cell line available in principle?
No
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA104132870

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2007-01-24
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Feeder cells human
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
L-Glutamine %
NEAA
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY

Other Genotyping (Cell Line)