chHES-90
The cell line is not validated yet.
NERCe002-A
General
Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
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External Databases (Donor) |
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BioSamples | SAMEA104132870 |
Ethics
Was the embryo established purely for research purposes? | Yes |
Have both parents consented to the use of the embryo for ESC derivation? | Yes |
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Alternative consent approval number | |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent expressly prevent the derivation of pluripotent stem cells? | No |
How may genetic information associated with the cell line be accessed? | |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | the ethical committee of Reproductive and Genetic Hospital of CITIC-Xiangya |
Approval number | 2001-01 |
hESC Derivation
Date of derivation | 2007-01-24 |
Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
PGD Embryo? |
No |
Culture Conditions
Feeder cells |
human |
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Passage method | Mechanically | ||||||||||||
O2 Concentration | 5 % | ||||||||||||
CO2 Concentration | 5 % | ||||||||||||
Medium |
Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Alkaline Phosphatase |
Yes |
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Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XY
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Other Genotyping (Cell Line) |
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