chHES-90

General#

Cell Line

hPSCreg Name NERCe002-A
Alternative name(s)
chHES-90
Cell line type Human embryonic stem cell (hESC)
Last update 20th October 2017
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Provider

Generator National Engineering and Research Center of Human Stem Cell (NERC)
Owner National Engineering and Research Center of Human Stem Cell (NERC)

External Databases

BioSamples SAMEA104132871
Cellosaurus CVCL_B008

General Information

Publications
Is the cell line available in principle?
No
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA104132870

Ethics#

Was the embryo established purely for research purposes? Yes
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the ethical committee of Reproductive and Genetic Hospital of CITIC-Xiangya
Approval number 2001-01

hESC Derivation#

Date of derivation 2007-01-24
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Feeder cells human
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
L-Glutamine %
NEAA
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY

Other Genotyping (Cell Line)