Establishment and characterization of a human embryonic stem cell line, NERCe002-A-3, with inducible 14-3-3ζ overexpression


NERCe002-A-3 cells were generated from the normal human embryonic stem cell line NERCe002-A. NERCe002-A-3 cells overexpressed 14-3-3ζ after exposure to doxycycline. 14-3-3ζ protein have the ability to bind a multitude of functionally diverse signalling proteins. The NERCe002-A-3 cell line is considered a model for functional studies of the 14-3-3ζ protein in hESC self-renewal and cell differentiation. Doxycycline-treated NERCe002-A-3 cells showed a>27-fold increase in relative expression of 14-3-3ζ as compared with un-induced cells. Characterization assays proved that NERCe002-A-3 cells express typical markers of pluripotency and have the ability to form the three germ layers in vivo. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors He J, Duan X, Li W, Peng Y, Yu J, Hu L, Zeng S, Wang Y, Lu G, Lin G, Sun Y
Journal Stem cell research
Publication Date 2018 Apr;28:11-15
PubMed 29414411
DOI 10.1016/j.scr.2018.01.017

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