Establishment and characterization of a human embryonic stem cell line, NERCe002-A-3, with inducible 14-3-3ζ overexpression
Summary
NERCe002-A-3 cells were generated from the normal human embryonic stem cell line NERCe002-A. NERCe002-A-3 cells overexpressed 14-3-3ζ after exposure to doxycycline. 14-3-3ζ protein have the ability to bind a multitude of functionally diverse signalling proteins. The NERCe002-A-3 cell line is considered a model for functional studies of the 14-3-3ζ protein in hESC self-renewal and cell differentiation. Doxycycline-treated NERCe002-A-3 cells showed a>27-fold increase in relative expression of 14-3-3ζ as compared with un-induced cells. Characterization assays proved that NERCe002-A-3 cells express typical markers of pluripotency and have the ability to form the three germ layers in vivo. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | He J, Duan X, Li W, Peng Y, Yu J, Hu L, Zeng S, Wang Y, Lu G, Lin G, Sun Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 Apr;28:11-15 |
| PubMed | 29414411 |
| DOI | 10.1016/j.scr.2018.01.017 |