General

Cell Line
hPSCreg name	NTUe003-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	NTUe003-A (RRID:CVCL_C110)
Alternative name(s)	NTU3
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	22nd May 2019
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Provider
Generator	National Taiwan University (NTU) Contact: Reproductive Medicine
Derivation country	Taiwan
External Databases
Cellosaurus	CVCL_C110
Wikidata	Q54931137
General Information
Publications	Chen HF et al. Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation. Human reproduction (Oxford, England). 2007 Feb;22(2):567-77.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Phenotype and Disease related information (Donor)
Diseases

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent

hESC Derivation

Embryo stage	Blastula with ICM and Trophoblast
ZP removal technique	Enzymatic

Culture Conditions

Feeder cells	MEF Cellfinder Ont Id: EFO_0004040
Passage method	Enzymatic, Mechanical
Medium	Other medium: Base medium: High glucose DMEM Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements Supplement Amount Unit L-Glutamine LIF

Characterisation

No characterisation data could be found.

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)