Generation of a human SOX10 knock-in reporter iPSC line for visualization of neural crest cell differentiation
Summary
SOX10 (SRY-box transcription factor 10) is not only a definitive molecular marker of neural crest cells (NCCs) but also an essential transcription factor for the differentiation of NCCs in vertebrate embryogenesis. Here, we report the establishment of a human SOX10 knock-in reporter iPSC line (SOX10-tdT) by CRISPR/Cas9-mediated homologous recombination, in which the expression of SOX10 can be monitored as tdTomato fluorescence. This iPSC line can provide a useful tool to model the differentiation process of human NCCs in vitro. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Yamashita T, Kamiya D, Kawaraichi N, Toyokawa H, Akaboshi T, Ikeya M, Toyooka Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Apr;60:102696 |
| PubMed | 35144211 |
| DOI | 10.1016/j.scr.2022.102696 |