Generation of a human SOX10 knock-in reporter iPSC line for visualization of neural crest cell differentiation


SOX10 (SRY-box transcription factor 10) is not only a definitive molecular marker of neural crest cells (NCCs) but also an essential transcription factor for the differentiation of NCCs in vertebrate embryogenesis. Here, we report the establishment of a human SOX10 knock-in reporter iPSC line (SOX10-tdT) by CRISPR/Cas9-mediated homologous recombination, in which the expression of SOX10 can be monitored as tdTomato fluorescence. This iPSC line can provide a useful tool to model the differentiation process of human NCCs in vitro. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Yamashita T, Kamiya D, Kawaraichi N, Toyokawa H, Akaboshi T, Ikeya M, Toyooka Y
Journal Stem cell research
Publication Date 2022 Apr;60:102696
PubMed 35144211
DOI 10.1016/j.scr.2022.102696

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