Generation of a homozygous ARX nuclear CFP (ARX(nCFP/nCFP)) reporter human iPSC line (HMGUi001-A-4)
Summary
The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3' fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARXnCFP/nCFP reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon+ α-like cells. Thus, ARXnCFP/nCFP iPSC line will provide a powerful tool to monitor human α-cell progenitor differentiation as well as ARX+ α-like cell function in vitro. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Moya N, Shahryari A, Burtscher I, Beckenbauer J, Bakhti M, Lickert H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Jul;46:101874 |
| PubMed | 32544857 |
| DOI | 10.1016/j.scr.2020.101874 |