Generation of integration-free induced pluripotent stem cells from a patient with spina bifida


A skin biopsy was obtained from a 14-year-old female patient with a history of Myelomeningocele. Dermal fibroblasts were isolated and reprogrammed with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. The generated induced Pluripotent Stem Cell (iPSC) clones NTDi4_09A were free of genomically integrated reprogramming genes, had a stable normal karyotype and expressed pluripotency markers. The iPSCs formed teratomas in mice, which were differentiated towards derivatives of the three germ layers in vivo. This iPSC line offers a useful resource to study a genetic profile of a patient with spina bifida. Copyright © 2018. Published by Elsevier B.V.

Authors Wang H, Zhao S, Finnell RH, George T, Cooney AJ
Journal Stem cell research
Publication Date 2018 Aug;31:27-30
PubMed 30007220
DOI 10.1016/j.scr.2018.05.007

Research Projects

Cell Lines