HAD-C 107 cGMP-grade, xeno-free Single-cell, Feeder-free Master Cell Bank

General#

Cell Line

hPSCreg Name HADe013-A
Alternative name(s)
HAD-C 107 cGMP-grade, xeno-free Single-cell, Feeder-free Master Cell Bank
Cell line type Human embryonic stem cell (hESC)
Last update 29th January 2020
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Provider

Generator Hadassah University Hospital (HAD)
Owner Hadassah Human Embryonic Stem Cell Research Center
Distributors
Derivation country Israel

External Databases

BioSamples SAMEA6592709
Cellosaurus CVCL_WW12
NIHhESC NIHhESC-20-0414

General Information

Notes This cell line is cGMP-grade, xeno-free, single-cell and feeder-free. It was originally derived as cell-fragments on umbilical-cord fibroblasts, then expanded as feeder-free. Contact us for the matching research-grade cell line information.
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: not allowed
Clinical: allowed
Commercial: allowed
Additional restrictions:

Research-use is only allowed if it will lead to clinical transplantations. Contact us for more information about matching research-grade cell line information. Ethical restrictions apply.

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history health history known; donors are healthy
Is the medical history available upon request? brief donor health summary available upon request

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA6592710

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number 0414
Has the donor agreed to be re-contacted? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Hadassah IRB Committee
Approval number 33-26.07.02
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Hadassah ESCRO Committee
Approval number N/A

hESC Derivation#

Date of derivation 2009-02-17
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Derived under xeno-free conditions?
Yes
Derivation under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

Feeder cells
No
Medium Other medium:
Base medium:
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
TRA 1-81
Yes
hESC Doubling Time (Potency)
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Alpha Fetoprotein
Ont Id: Orphanet_173230
In vitro directed differentiation
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Actin, Aortic Smooth Muscle
Ont Id: NCIT_C103972
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Tubulin Beta 3 Class III
Ont Id: Orphanet_226013
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal Male Karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)