iso01LUMC0110iALK10
LUMCi030-B-1
General
Cell Line |
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| hPSCreg name | LUMCi030-B-1 |
| Cite as: | LUMCi030-B-1 (RRID:CVCL_ZL48) |
| Alternative name(s) |
iso01LUMC0110iALK10
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 19th March 2020 |
| User feedback | |
Provider |
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| Generator | Leiden University Medical Center (LUMC) |
External Databases |
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| BioSamples | SAMEA6878128 |
| Cellosaurus | CVCL_ZL48 |
| Wikidata | Q98127105 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA6712311 |
Ethics
Also have a look at the ethics information for the parental line
LUMCi030-B
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
LUMCi030-B.
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| Passage number reprogrammed | 3 |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Plasmid |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
TeSR™ E8™
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
Morphology pictures
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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