Ma01
ACTe001-A
General
Cell Line |
|
| hPSCreg name | ACTe001-A |
| Cite as: | ACTe001-A (RRID:CVCL_C341) |
| Alternative name(s) |
Ma01
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator | Advanced Cell Technology, Inc. - Cellular Reprogramming unit (ACT) |
| Derivation country | United States |
External Databases |
|
| Cellosaurus | CVCL_C341 |
| Wikidata | Q54903874 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Cleavage (Mitosis) |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Chemical |
| Cell isolation | Mechanical |
| Cell seeding | Single cell and ICM isolated |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
| Passage method | Enzymatic, Enzymatic, Mechanical |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: DMEM
Main protein source: Fetal bovine serum Serum concentration: 10 % |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
|
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
|
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| TRA 1-81 |
Yes |
|
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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