General#

Cell Line

hPSCreg Name IDISi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 25th May 2018
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Provider

Generator Health Research Institute of Santiago de Compostela (IDIS)
Owner Health Research Institute of Santiago de Compostela (IDIS)

External Databases

Cellosaurus CVCL_RV23
BioSamples SAMEA4675695

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
CADASIL
The donor is a carrier of a disease-associated mutation.
Synonyms
  • Hereditary multi-infarct dementia
  • Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

External Databases (Donor)

BioSamples SAMEA4675696

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR
Notes on reprogramming vector detection Detection of the DNA vector by PCR of a sample of PBMCs 24h after infection. Absence of the vector was checked by PCR of several clones of the new line at passage 5
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones For the first and second passages, colonies were selected and pickedwith the help of an Origio Stripper (Origio)with Stripper tips (Origio) of 175 μm in diameter. Later passages were performed mechanically, using cell lifters.
Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Supplements
mTeSR1 Supplemental mix 100 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
Morphology
EBs 05-05-17 HF_Image018.tif
FOXA2 (Green) + DAPI (Blue)
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Vimentin
Unkown
Morphology
EBs 05-05-17 HF_Image032.tif
Vimentin (Green) + DAPI (Blue)
EBs 05-05-17 HF_Image028.tif
Vimentin (Green) + DAPI (Blue)
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Nestin
Unkown
Morphology
EBs 19-05-17_Image012.tif
Nestin (Green) + DAPI (Blue)
EBs 19-05-17_Image025.tif
Nestin (Green) + DAPI (Blue)

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)