POWHe001-A-2

E1C2

General#

Cell Line

hPSCreg Name POWHe001-A-2
Alternative name(s)
E1C2
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Prince of Wales Hospital (POWH)
Derivation country Australia

External Databases

Cellosaurus CVCL_6968

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Also have a look at the ethics information for the parental line POWHe001-A .
Alternatives to consent

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Mechanical

Culture Conditions#

Feeder cells Human Fetal Fibroblast
Cellfinder Ont Id: FMA_63877

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GATA4
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)

Genetic Modification#