E1C3	
    			    			
                POWHe001-A-3            
            
        General
| Cell Line | |
| hPSCreg name | POWHe001-A-3 | 
| Cite as: | POWHe001-A-3 (RRID:CVCL_6969) | 
| Alternative name(s) | 
	E1C3	 | 
| Cell line type | Human embryonic stem cell (hESC) | 
| Similar lines | |
| Last update | 22nd May 2019 | 
| User feedback | |
| Provider | |
| Generator | Prince of Wales Hospital (POWH) 
    									Contact:
    									 Embryonic Stem Cells group | 
| Derivation country | Australia | 
| External Databases | |
| Cellosaurus | CVCL_6969 | 
| Wikidata | Q54831653 | 
| General Information | |
| Publications | |
| * Is the cell line readily obtainable for third parties? | No | 
| Subclone of | |
Donor Information
| General Donor Information | |
| Sex | female | 
| Phenotype and Disease related information (Donor) | |
| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
        				
		
			POWHe001-A		
        				.
        			        
        			        			        			        			| Alternatives to consent | 
hESC Derivation
| The source cell information can be found in the parental cell line
							POWHe001-A. | 
Culture Conditions
| Feeder cells | Human Fetal Fibroblast Cellfinder Ont Id: FMA_63877 | 
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles | 
| GATA4 | Yes |  | ||||
| NANOG | Yes |  | ||||
| POU5F1 (OCT-4) | Yes |  | ||||
| Alkaline Phosphatase | Yes |  | ||||
| SSEA-3 | Yes |  | ||||
| SSEA-4 | Yes |  | ||||
| TRA 1-60 | Yes |  | ||||
| TRA 1-81 | Yes |  | 
Differentiation Potency
Genotyping
| Karyotyping (Cell Line) | |
| Has the cell line karyotype been analysed? | Yes 
												46XX,+15,-17
											 | 
| Other Genotyping (Cell Line) | |

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