PUMCHi019-A

General

Cell Line

hPSCreg Name
PUMCHi019-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 10th February 2022
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Provider

Generator Peking Union Medical College Hospital (PUMCH)

External Databases

BioSamples SAMEA12789792
CLO CLO_0103707

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Autosomal dominant optic atrophy plus syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • DOA+
  • Optic atrophy-deafness-polyneuropathy-myopathy syndrome
  • Optic atrophy-hearing loss-polyneuropathy-myopathy syndrome

External Databases (Donor)

BioSamples SAMEA12789793

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Peking Union Medical College Hospital
Approval number JS-2059
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Mononuclear
Possessing a single nucleus.
Synonyms
  • Mononuclear

Reprogramming method

Vector type Integrating
Vector Virus ()
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Self-renewal
Unknown
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)