FiPS Ctrl1-SV4F-7
ESi044-C
General#
Cell Line |
|
hPSCreg Name | ESi044-C |
Alternative name(s) |
FiPS Ctrl1-SV4F-7
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
|
Generator | Spanish Stem Cell Bank (ES) |
Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
Distributors | |
Derivation country | Spain |
External Databases |
|
BioSamples | SAMEA104129970 |
Cellosaurus | CVCL_9S19 |
General Information |
|
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
|
Sex | male |
Ethnicity | caucasian |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Family history | no |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA104008501 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
|
Source cell type |
foreskin fibroblastFibroblast from foreskin.
Synonyms
|
Source cell origin |
prepuce of penisA retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
|
Age of donor (at collection) | 5-9 |
Collected in | 2014 |
Passage number reprogrammed | 3 |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
|
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Other |
|
Selection criteria for clones | good morphology and absence of virus |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium | mTeSR™ 1 |
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|||||
SSEA-3 |
Yes |
|||||
NANOG |
Yes |
|||||
TRA 1-81 |
Yes |
|||||
SOX2 |
Yes |
|||||
SSEA-4 |
Yes |
|||||
TRA 1-60 |
Yes |
|||||
Alkaline Phosphatase |
Yes |
|
|
Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
Marker | Expressed |
TUJI1 |
Yes |
GFAP |
Yes |
Microbiology / Virus Screening |
|
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
Login to share your feedback, experiences or results with the research community.