Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor


Psoriasis is a chronic inflammatory skin disease that speeds up the life cycle of skin cells, forming scales and red patches that are itchy and sometimes painful. It is a complex disease of autoimmune origin and genetic predisposition with more than 10 different loci associated. Here we described the production of an iPSC line generated by Sendai Virus (Klf4, Oct3/4, Sox2 and c-Myc) reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) from a Psoriasis patient. The iPSC line generated has normal 46XY karyotype, is free of SeV genome and transgenes insertions, express high levels of pluripotency markers and can differentiate into all three germ layers. Copyright © 2020. Published by Elsevier B.V.

Authors Vallejo-Diez S, Fleischer A, María Martín-Fernández J, Sánchez-Gilabert A, Gómez-Martínez C, Castresana M, Bachiller D
Journal Stem cell research
Publication Date 2020 Jul 17;47:101917
PubMed 32707489
DOI 10.1016/j.scr.2020.101917

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