LRB04
KUe004-A
General
Cell Line |
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| hPSCreg name | KUe004-A |
| Cite as: | KUe004-A (RRID:CVCL_B239) |
| Alternative name(s) |
LRB04
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
University of Copenhagen (KU)
Contact:
Laboratory of Reproductive Biology |
| Derivation country | Denmark |
External Databases |
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| Cellosaurus | CVCL_B239 |
| Wikidata | Q54903012 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
Mouse Embryonic Fibroblast Cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Mechanically | ||||||
| O2 Concentration | 7 % | ||||||
| CO2 Concentration | 6 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| GATA6 |
Yes |
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| PAX6 |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| STELLA |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 25
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Other Genotyping (Cell Line) |
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