Generation of TSC1 knockout induced pluripotent stem cell (iPSC) line


The TSC1 gene is a tumor suppressor gene that encodes for the growth inhibitory protein hamartin. It was founded clinically relevant to tuberous sclerosis complex (TSC) and related epilepsy. Variants in TSC1 resulted in tuberous sclerosis, focal cortical dysplasia (FCD) Type II, pulmonary lymphangioleiomyomatosis and change in everolimus sensitivity. Here, we generated induced pluripotent stem cells (iPSC) from a normal individual by electroporation of peripheral blood mononuclear cells (PBMC), and further generated TSC1-knockout human iPSC line via CRISPR/Cas9 gene editing. The resulting iPSCs had normal karyotype, free of genomically integrated epitomal plasmids, expressed pluripotency markers, and maintained trilineage differentiation potential. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Shan W, Yang H, Ren Q, Wang Q, An G
Journal Stem cell research
Publication Date 2022 Aug 5;64:102888
PubMed 35961105
DOI 10.1016/j.scr.2022.102888

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