Generation of a MIR5004 knockout cell line from human induced pluripotent stem cells by CRISPR/Cas9 gene editing
Summary
MIR5004 is located in the intronic region of SYNGAP1, a genetic risk factor for Autism Spectrum Disorders (ASD), and co-expressed with SYNGAP1 in brain tissue, which indicates that MIR5004 may play an important role in ASD pathogenesis through the regulation of SYNGAP1. Here, we generated a MIR5004 knockout human induced pluripotent stem cell (iPSC) line SHCDCLi001-B. SHCDCLi001-B shows expression of pluripotent markers, three lineage differentiation capacity, normal morphology and karyotypes, the same DNA origin with wild type iPSC (iPSC-WT) and no off-target effects, making it as a valuable tool for studying the interplay between MIR5004 and SYNGAP1 in ASD pathogenesis. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Gulimiheranmu M, Li S, Zhou J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Jul;62:102805 |
| PubMed | 35576811 |
| DOI | 10.1016/j.scr.2022.102805 |