Generation of heterozygous (MRli003-A-3) and homozygous (MRli003-A-4) TRPM4 knockout human iPSC lines


TRPM4 is a Ca2+-activated channel mediating the transport of monovalent cations across the cell membrane. Mutations in the TRPM4 gene have been associated with cardiac arrhythmias in humans. Using CRISPR/Cas9 gene editing technology, we established two TRPM4 knockout human iPSC lines - one heterozygous (MRli003-A-3) and one homozygous (MRli003-A-4) - by inserting a frameshift mutation in exon 2 of the TRPM4 gene. Both lines maintained pluripotency, a normal karyotype, parental cell morphology, and the ability to differentiate into the three germ layers. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang F, Meier AB, Lipp P, Laugwitz KL, Dorn T, Moretti A
Journal Stem cell research
Publication Date 2022 Apr;60:102731
PubMed 35245852
DOI 10.1016/j.scr.2022.102731

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