Generation of induced pluripotent stem cell line (ZZUi030-A) from a patient with spastic paraplegia type 7


Spastic paraplegia type 7 is a rare and classical monogenic inherited neurodegenerative disease caused by heterozygous mutations in the SPG7 gene. The principle clinical features include progressive spasms of the lower limbs, scissor gait, and muscle weakness. The disease currently has no effective treatment. In this study, we obtained dermal fibroblasts from a patient, which were successfully transformed into induced pluripotent stem cells (iPSCs) by employing reprogramming plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and L-MYC. Our method provides a resource for mechanism exploration, drug research, cell transplantation, and gene therapy. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang Q, Wang Z, Sun H, Chen N, Xu X, Yang J, Gao Y, Wang Y, Xu Y
Journal Stem cell research
Publication Date 2021 Oct;56:102525
PubMed 34509920
DOI 10.1016/j.scr.2021.102525

Research Projects

Cell Lines