CHOPACTG2-R257C

General

Cell Line

hPSCreg name CHOPi012-A
Cite as:
CHOPi012-A (RRID:CVCL_C6SS)
Alternative name(s)
CHOPACTG2-R257C
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 28th July 2023
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Provider

Generator The Children's Hospital of Philadelphia (CHOP)
Owner The Children's Hospital of Philadelphia (CHOP)
Distributors
Derivation country United States

External Databases

Cellosaurus CVCL_C6SS
Wikidata Q117704241
BioSamples SAMEA114192292

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
visceral myopathy” (VSCM1) also caused by mutations in ACGT2
The donor is a carrier of a disease-associated mutation and affected.

External Databases (Donor)

BioSamples SAMEA114192405

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Robert Heuckeroth, MD, heuckerothr@chop.edu
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Robert Heuckeroth, MD, heuckerothr@chop.edu
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? CHOP IRB
Approval number IRB 13-010357
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Mononuclear cells collected from peripheral blood.
Synonyms
  • Peripheral Blood Mononuclear cells
  • Derived Peripheral Blood Mononuclear Cell

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: DMEMF12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Passage number: 14

Other Genotyping (Cell Line)