Generation of induced pluripotent stem cell lines from two Neuroblastoma patients carrying a germline ALK R1275Q mutation


Neuroblastoma (NB) is an embryonic tumor of the peripheral nervous system and one of the most common solid cancers in infants. Mutations in the Anaplastic lymphoma tyrosine kinase (ALK) gene are common in NB. To study the contribution of ALK mutations in NB initiation and progression, we reprogrammed fibroblasts from two related NB patients carrying germline mutations in ALK (R1275Q) using non-integrating Sendai virus. The iPS cells are grown in a feeder- and xeno-free conditions, have normal karyotype, retain the ALK R1275Q mutation, have been characterized by expression of pluripotency markers and differentiation to all three germ layers. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Marin Navarro A, Day K, Kogner P, Wilhelm M, Falk A
Journal Stem cell research
Publication Date 2019 Jan;34:101356
PubMed 30605844
DOI 10.1016/j.scr.2018.11.017

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