201B7
KUIFMSi004-C
General
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7996630 |
Ethics
Also have a look at the ethics information for the parental line
KUIFMSi004-A
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| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
KUIFMSi004-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
SNL76/7 Cellfinder Ont Id: CELDA_00013400 |
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| Passage method | Mechanically | ||||||
| Medium |
Other medium:
Base medium: Primate ES medium
Main protein source: Serum concentration: 10 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| E-Cadherin |
Yes |
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| SALL4 |
Yes |
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| DPPA4 |
Yes |
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| TERT |
Yes |
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| LIN28 |
Yes |
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| foxd3 |
Yes |
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| KLF4 |
Yes |
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| MYC |
Yes |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| nodal |
Yes |
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| podxl |
Yes |
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| LEFTY1 |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| FGF4 |
Yes |
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| Dppa5 |
Yes |
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| DPPA2 |
Yes |
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| GABRB3 |
Yes |
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| TDGF1 |
Yes |
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| GAL |
Yes |
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| IFITM1 |
Yes |
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| UTF1 |
Yes |
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| GRB7 |
Yes |
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| CD9 |
Yes |
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| BRIX1 |
Yes |
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| NAT1 |
Yes |
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Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
| Marker | Expressed |
| AFP |
Yes |
| FOXA2 |
Yes |
| Keratin 8 |
Yes |
| Keratin 18 |
Yes |
| SOX17 |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| TNNT2 |
Yes |
| MEF2C |
Yes |
| MYL7 |
Yes |
| MYH7B |
Yes |
| NKX2-5 |
Yes |
| Actin, alpha 2, smooth muscle |
Yes |
| Desmin |
Yes |
| MSX1 protein |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| Tyrosine hydroxylase |
Yes |
| class III beta tubulin |
Yes |
| DDC |
Yes |
| DAT |
Yes |
| CHAT |
Yes |
| LMX1B |
Yes |
| MAP2 |
Yes |
| glial fibrillary acidic protein |
Yes |
| PAX6 |
Yes |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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