KUIFMSi004-B

201B6

General#

Cell Line

hPSCreg Name KUIFMSi004-B
Alternative name(s)
201B6
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th July 2017
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Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_A065

General Information

Publications
Is the cell line available in principle?
No
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

Other cell lines of this donor

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line KUIFMSi004-A.

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells SNL76/7
Cellfinder Ont Id: CELDA_00013400
Passage method Mechanically
Medium Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source:
Serum concentration: 10 %
Supplements
hrbFGF 4 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
E-Cadherin
Yes
SALL4
Yes
DPPA4
Yes
TERT
Yes
LIN28
Yes
foxd3
Yes
KLF4
Yes
c-MYC
Yes
DNMT3B
Yes
GDF3
Yes
nodal
Yes
podxl
Yes
LEFTY1
Yes
ZFP42 (REX-1)
Yes
FGF4
Yes
Dppa5
Yes
DPPA2
Yes
GABRB3
Yes
TGDF1
Yes
GAL
Yes
IFITM1
Yes
UTF1
Yes
GRB7
Yes
CD9
Yes
BRIX1
Yes
MYC
Yes
NAT1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
FOXA2
Yes
Keratin 8
Yes
Keratin 18
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
TNNT2
Yes
MEF2C
Yes
MYL7
Yes
MYH7B
Yes
NKX2-5
Yes
alpha-smooth muscle actin
Yes
Desmin
Yes
MSX1 protein
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
Tyrosine hydroxylase
Yes
class III beta tubulin
Yes
DDC
Yes
DAT
Yes
CHAT
Yes
LMX1B
Yes
MAP2
Yes
glial fibrillary acidic protein
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#