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KUIFMSi004-A-1

Registration Summary:

hPSC MPP8 Neon Green

KUIFMSi004-A-1

General

Cell Line
hPSCreg name KUIFMSi004-A-1
Cite as:
KUIFMSi004-A-1
Alternative name(s)
hPSC MPP8 Neon Green
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 21st November 2023
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Provider
Generator Pirogov Russian National Research Medical University (RNRMU)

External Databases
BioSamples SAMEA114266903

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA7996630

Ethics

Also have a look at the ethics information for the parental line KUIFMSi004-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General
The source cell information can be found in the parental cell line KUIFMSi004-A.
Passage number reprogrammed 10

Reprogramming method
Vector type None

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
Medium mTeSR™ 2
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
SSEA-4
Yes
Differentiation Potency
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Desmin
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
SOX2
Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46 XX
iPSCMPP8-Green_Karyo.png
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
This cell line KUIFMSi004-A-1(alternative name iPSC MPP8-Green) expresses the fragment of MPP8 in fusion with mNeonGreen. This transgene was made by lentiviral transduction. Cells expressed this fusion protein as a nulear localized flurescent reporter. I confirmed that this reporter differs this subclone from parental cell line KUIFMSi004-A. All features (including markers of pluripotency, STR markers and karyotyping) belong to this subclone iPSC MPP8-Green.
Viral
Lentivirus