201B2
KUIFMSi004-D
General
Cell Line |
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| hPSCreg name | KUIFMSi004-D |
| Cite as: | KUIFMSi004-D (RRID:CVCL_A063) |
| Alternative name(s) |
201B2
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
KUIFMSi004-A (201B1) KUIFMSi004-A-1 (hPSC MPP8 Neon Green) KUIFMSi004-E (201B3) KUIFMSi004-B (201B6) KUIFMSi004-C (201B7) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
Kyoto University (KUIMS)
Contact:
iPS Cell Research and Application |
| Derivation country | Japan |
External Databases |
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| Cellosaurus | CVCL_A063 |
| Wikidata | Q54583058 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7996630 |
Ethics
Also have a look at the ethics information for the parental line
KUIFMSi004-A
.
| Alternatives to consent |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
KUIFMSi004-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
SNL76/7 Cellfinder Ont Id: CELDA_00013400 |
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| Passage method | Mechanically | ||||||
| Medium |
Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source: Serum concentration: 10 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| E-Cadherin |
Yes |
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| SALL4 |
Yes |
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| DPPA4 |
Yes |
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| TERT |
Yes |
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| LIN28 |
Yes |
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| foxd3 |
Yes |
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| KLF4 |
Yes |
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| MYC |
Yes |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| nodal |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| LEFTY1 |
Yes |
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| FGF4 |
Yes |
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| Dppa5 |
Yes |
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| DPPA2 |
Yes |
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| GABRB3 |
Yes |
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| TDGF1 |
Yes |
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| GAL |
Yes |
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| IFITM1 |
Yes |
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| UTF1 |
Yes |
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| GRB7 |
Yes |
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| CD9 |
Yes |
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| BRIX1 |
Yes |
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| NAT1 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| troponin T type 2 |
Yes |
| myocyte enhancer factor 2C |
Yes |
| myosin, light chain 7, regulatory |
Yes |
| myosin, heavy chain 7B, cardiac muscle |
Yes |
| NK2 homeobox 5 |
Yes |
| Actin, alpha 2, smooth muscle |
Yes |
| Desmin |
Yes |
| MSX1 protein |
Yes |
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| Tyrosine hydroxylase |
Yes |
| class III beta tubulin |
Yes |
| aromatic L-amino acid decarboxylase |
Yes |
| Dopamine transporter |
Yes |
| choline O-acetyltransferase |
Yes |
| LIM homeobox transcription factor 1, beta |
Yes |
| microtubule-associated protein 2 |
Yes |
| glial fibrillary acidic protein |
Yes |
| MAP2 |
Yes |
| PAX6 |
Yes |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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