201B1
KUIFMSi004-A
General
Donor Information
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 35-39 |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7996630 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | permission from Spanish ministry in Catalan |
| Alternative consent approval number | |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Spanish ministry |
| Approval number | registre d’entrada núm.0336i /597/2019 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Spanish ministry |
| Approval number | registre d’entrada núm.0336i /597/2019 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Any skin fibroblast that is part of some dermis.
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| Source cell origin |
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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| Source cell line lot number |
Lot No.1388
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| Age of donor (at collection) | 35-39 |
| Source cell line vendor | Cell Application Inc |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
SNL76/7 Cellfinder Ont Id: CELDA_00013400 |
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| Passage method | Mechanically | ||||||
| Medium |
Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source: Serum concentration: 10 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| nodal |
Yes |
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| LEFTY1 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| FGF4 |
Yes |
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| Dppa5 |
Yes |
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| DPPA2 |
Yes |
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| DPPA4 |
Yes |
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| TERT |
Yes |
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| GABRB3 |
Yes |
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| TDGF1 |
Yes |
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| GAL |
Yes |
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| IFITM1 |
Yes |
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| UTF1 |
Yes |
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| GRB7 |
Yes |
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| CD9 |
Yes |
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| BRIX1 |
Yes |
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| KLF4 |
Yes |
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| MYC |
Yes |
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| NAT1 |
Yes |
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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