KUIFMSi004-A

201B1

General#

Cell Line

hPSCreg Name KUIFMSi004-A
Alternative name(s)
201B1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 20th January 2021
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Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_A062
CLO CLO_0100022
BioSamples SAMEA7996629

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 35-39
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7996630

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: We confirmed Cell Applications, Inc. that the copy is filed at the organization, which collected the original tissue, but because of personal information perspective, even Cell Applications, cannot be provided the documents.
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? Yes
Alternatives to consent permission from Spanish ministry in Catalan
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Spanish ministry
Approval number registre d’entrada núm.0336i /597/2019
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Spanish ministry
Approval number registre d’entrada núm.0336i /597/2019
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Source cell line lot number
Lot No.1388
Age of donor (at collection) 35-39
Source cell line vendor Cell Application Inc

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells SNL76/7
Cellfinder Ont Id: CELDA_00013400
Passage method Mechanically
Medium Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source:
Serum concentration: 10 %
Supplements
hrbFGF 4 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GDF3
Yes
nodal
Yes
LEFTY1
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
FGF4
Yes
Dppa5
Yes
DPPA2
Yes
DPPA4
Yes
TERT
Yes
GABRB3
Yes
TDGF1
Yes
GAL
Yes
IFITM1
Yes
UTF1
Yes
GRB7
Yes
CD9
Yes
BRIX1
Yes
KLF4
Yes
MYC
Yes
NAT1
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)