201B1
KUIFMSi004-A
General#
Cell Line |
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| hPSCreg Name | KUIFMSi004-A |
| Alternative name(s) |
201B1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 8th December 2017 |
| User feedback | |
Provider |
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| Generator |
Kyoto University (KUIMS)
Contact:
iPS Cell Research and Application |
| Derivation country | Japan |
External Databases |
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| Cellosaurus | CVCL_A062 |
General Information |
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| Publications | |
| Is the cell line available in principle? |
No |
| Subclones | |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Donor Relations |
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| Other cell lines of this donor | |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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| Source cell type |
fibroblast of dermis |
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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| Age of donor (at collection) | 35-39 |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unkown |
| Absence of reprogramming vector(s)? |
Unkown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Unkown |
| Derived under GMP? |
Unkown |
| Available as clinical grade? |
Unkown |
Culture Conditions#
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
SNL76/7 Cellfinder Ont Id: CELDA_00013400 |
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| Passage method | Mechanically | ||||||
| Medium |
Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source: Serum concentration: 10 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| nodal |
Yes |
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| LEFTY1 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| FGF4 |
Yes |
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| Dppa5 |
Yes |
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| DPPA2 |
Yes |
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| DPPA4 |
Yes |
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| TERT |
Yes |
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| GABRB3 |
Yes |
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| TDGF1 |
Yes |
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| GAL |
Yes |
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| IFITM1 |
Yes |
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| UTF1 |
Yes |
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| GRB7 |
Yes |
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| CD9 |
Yes |
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| BRIX1 |
Yes |
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| KLF4 |
Yes |
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| MYC |
Yes |
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| NAT1 |
Yes |
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Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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