201B1
KUIFMSi004-A
General
Cell Line |
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| hPSCreg Name | KUIFMSi004-A |
| Alternative name(s) |
201B1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 20th January 2021 |
| User feedback | |
Provider |
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| Generator |
Kyoto University (KUIMS)
Contact:
iPS Cell Research and Application |
| Derivation country | Japan |
External Databases |
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| Cellosaurus | CVCL_A062 |
| CLO | CLO_0100022 |
| BioSamples | SAMEA7996629 |
| Wikidata | Q54583056 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclones | |
Donor Information
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 35-39 |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7996630 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | We confirmed Cell Applications, Inc. that the copy is filed at the organization, which collected the original tissue, but because of personal information perspective, even Cell Applications, cannot be provided the documents. |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | permission from Spanish ministry in Catalan |
| Alternative consent approval number | |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Spanish ministry |
| Approval number | registre d’entrada núm.0336i /597/2019 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Spanish ministry |
| Approval number | registre d’entrada núm.0336i /597/2019 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell type |
fibroblast of dermis |
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
| Source cell line lot number |
Lot No.1388
|
| Age of donor (at collection) | 35-39 |
| Source cell line vendor | Cell Application Inc |
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
SNL76/7 Cellfinder Ont Id: CELDA_00013400 |
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| Passage method | Mechanically | ||||||
| Medium |
Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source: Serum concentration: 10 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| nodal |
Yes |
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| LEFTY1 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| FGF4 |
Yes |
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| Dppa5 |
Yes |
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| DPPA2 |
Yes |
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| DPPA4 |
Yes |
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| TERT |
Yes |
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| GABRB3 |
Yes |
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| TDGF1 |
Yes |
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| GAL |
Yes |
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| IFITM1 |
Yes |
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| UTF1 |
Yes |
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| GRB7 |
Yes |
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| CD9 |
Yes |
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| BRIX1 |
Yes |
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| KLF4 |
Yes |
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| MYC |
Yes |
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| NAT1 |
Yes |
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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