KUIFMSi004-E

201B3

General#

Cell Line

hPSCreg Name KUIFMSi004-E
Alternative name(s)
201B3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_A064

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

Other cell lines of this donor

Ethics#

Also have a look at the ethics information for the parental line KUIFMSi004-A .
Alternatives to consent

hIPSC Derivation#

General

The source cell information can be found in the parental cell line KUIFMSi004-A.

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells SNL76/7
Cellfinder Ont Id: CELDA_00013400
Passage method Mechanically
Medium Other medium:
Base medium: Primate ES medium
Main protein source:
Serum concentration: 10 %
Supplements
hrbFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GDF3
Yes
nodal
Yes
ZFP42 (REX-1)
Yes
podxl
Yes
LEFTY1
Yes
POU5F1
Yes
SOX2
Yes
NANOG
Yes
FGF4
Yes
Dppa5
Yes
DPPA2
Yes
DPPA4
Yes
TERT
Yes
GABRB3
Yes
TDGF1
Yes
GAL
Yes
IFITM1
Yes
UTF1
Yes
GRB7
Yes
CD9
Yes
BRIX1
Yes
KLF4
Yes
MYC
Yes
NAT1
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#