BT2-UCiPS4F1, BT-iPSCs 2
The cell line is not validated yet.
ESi088-A
General
Cell Line |
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| hPSCreg name | ESi088-A |
| Cite as: | ESi088-A (RRID:CVCL_C1PQ) |
| Alternative name(s) |
BT2-UCiPS4F1, BT-iPSCs 2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 17th October 2025 |
| User feedback | |
Provider |
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| Generator | Spanish Stem Cell Bank (ES) |
| Derivation country | Spain |
External Databases |
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| BioSamples | SAMEA110466389 |
| Cellosaurus | CVCL_C1PQ |
| Wikidata | Q114311379 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA110466420 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Alternatives to consent are available? | No |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | Unknown |
| Has the donor been informed about how her/his data will be protected? | No |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| * Does consent pertain to a specific research project? | Yes |
| Details on restriction to research project | The consent describes a specific use of the derived pluripotent stem cells as a neuroprotective therapy against the radiotherapy-reurological sequelae in an experimental model of childhood cancer. |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Andalusian Ethics Committee of Research with embryonic samples and other similar cells |
| Approval number | PR-03-2020 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Andalusian Ethics Committee of Research with embryonic samples and other similar cells |
| Approval number | PR-03-2020 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Thermo Fisher Scientific |
hIPSC Derivation
General |
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| Source cell type |
Any of the cells of the epithelium, which covers the body and lines its cavities and glands.
Synonyms
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| Source cell origin |
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
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| Passage number reprogrammed | 5 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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| Notes on reprogramming vector detection | As it is reported for UDC-derived iPSC, Sendai reprogramming vectors become undetectable in this cell type, normally at passages higher than 20 (Stem Cell Res. 2017 Jan;18:41-44, Stem Cell Res. 2020 Aug;47:101886). |
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
No |
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| Passage method |
Enzyme-free cell dissociation
ReLeSR™ (STEMCELL, CAT#05872)
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| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
mTeSR™ Plus
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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| TERT |
Yes |
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| Alkaline Phosphatase |
Yes |
Morphology pictures
BT2-UCiPS4F1_iPSC morphology.pdf
BT2-UCiPS4F1_iPSC morphology
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| AFP |
Yes |
Morphology
BT2-UCiPS4F1_Germ layers.pdf
BT2-UCiPS4F1_Germ layers
In vitro directed differentiation
| Marker | Expressed |
| Smooth muscle actin |
Yes |
Morphology
BT2-UCiPS4F1_Germ layers.pdf
BT2-UCiPS4F1_Germ layers
In vitro directed differentiation
| Marker | Expressed |
| Nestin |
Yes |
Morphology
BT2-UCiPS4F1_Germ layers.pdf
BT2-UCiPS4F1_Germ layers
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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