Generation of an induced pluripotent stem cell lines NSHDMUi001-A from patients with type 2 diabetes


Type 2 diabetes mellitus (T2DM) is common in China, and its aetiology and pathogenesis are still unclear. We reprogrammed pEP4EO2SEN2K and pEP4EO2SET2K, pCEP4-M2L was electrotransfected in T2DM patients with pEP4EO2SEN2K, and pCEP4-M2L was electrotransfected in T2DM patients expressing the OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT transcription factors to obtain induced pluripotent stem cells (iPSCs). The obtained iPSCs have been verified to have pluripotency, normal karyotype and differentiation potential; therefore, these cells can be used in the study of disease pathophysiology and drug development to create new therapeutic targets for T2DM and associated central nervous system damage. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Li P, Wang C, Wang J, Tan J, Liu Y, Lin Y
Journal Stem cell research
Publication Date 2023 Jun;69:103087
PubMed 37028179
DOI 10.1016/j.scr.2023.103087

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