Establishment of TUSMi009-A, an induced pluripotent stem cell (iPSC) line from a 24-year-old Chinese Han patient with gliocytoma
Summary
Glioblastoma is the most common malignant primary brain tumor with poor prognosis and low long-term survival rate. Here we described the production of an iPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 24-year-old female gliocytoma patient. The PBMCs were reprogrammed by the non-integrating Sendai Virus with human OKSM (OCT3/4, SOX2, KLF4, and c-MYC) transcription factors. The generated iPSCs were positive for pluripotent stem cell markers demonstrated by immunocytochemistry. Besides, they displayed a normal karyotype and had the potential to differentiate spontaneously three germ layers in vitro. Our model might be useful in studying the pathogenesis of gliocytoma patients. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Chen K, Zhuang Z, Wang Q, Li K, Xu S, Liu M, Zhang K, Wei L, Zhang Y, Lian H, Zhang B, Zhong C |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Oct;56:102546 |
| PubMed | 34587579 |
| DOI | 10.1016/j.scr.2021.102546 |