Establishment of TUSMi009-A, an induced pluripotent stem cell (iPSC) line from a 24-year-old Chinese Han patient with gliocytoma

Summary

Glioblastoma is the most common malignant primary brain tumor with poor prognosis and low long-term survival rate. Here we described the production of an iPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 24-year-old female gliocytoma patient. The PBMCs were reprogrammed by the non-integrating Sendai Virus with human OKSM (OCT3/4, SOX2, KLF4, and c-MYC) transcription factors. The generated iPSCs were positive for pluripotent stem cell markers demonstrated by immunocytochemistry. Besides, they displayed a normal karyotype and had the potential to differentiate spontaneously three germ layers in vitro. Our model might be useful in studying the pathogenesis of gliocytoma patients. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Chen K, Zhuang Z, Wang Q, Li K, Xu S, Liu M, Zhang K, Wei L, Zhang Y, Lian H, Zhang B, Zhong C
Journal Stem cell research
Publication Date 2021 Oct;56:102546
PubMed 34587579
DOI 10.1016/j.scr.2021.102546

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