Efficient generation of induced pluripotent stem cell lines from healthy donors' peripheral blood mononuclear cells of different genders
Summary
Peripheral blood mononuclear cell (PBMC) are recognized as a conveniently collected reprogramming resource. Several methods are available in academia to reprogram PBMC into induced pluripotent stem cells (iPSC). In this research, we reprogrammed PBMC of different genders by using non-integrative non-viral liposome electrotransfer containing the reprogramming factors OCT4, SOX2, KLF4, and c-MYC. The three obtained iPSC cell lines were karyotypically normal and showed significant tritiated differentiation potential in vitro and in vivo. Our study provided an efficient procedure for reprogramming PBMC into iPSC and obtained three well-functioning iPSC, that may contribute to advance personalized cell therapy in the future. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Gao Y, Cheng Y, Lin B, Xiao D, Wen J, Xu J, Zheng R, Zhang M, Cai C, Hu J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103421 |
| PubMed | 38636268 |
| DOI | 10.1016/j.scr.2024.103421 |