Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient


Skin fibroblasts and tumor fibroblasts were extracted from a 64-year old male patient clinically diagnosed with laryngeal carcinoma. Control and tumor specific induced pluripotent stem cells were reprogrammed with 5 reprogramming factors, Klf-4, c-Myc, Oct-4, Sox-2, and Lin-28, using the messenger RNA reprogramming system. The transgene-free iPSC lines showed pluripotency, confirmed by immunofluorescence staining. The iPSC lines also showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This in vitro cellular model can be used to study the oncogenesis and pathogenesis of laryngeal carcinoma. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang Y, Zhu J, Dai Y, Wang L, Liu R
Journal Stem cell research
Publication Date 2017 Dec;25:283-285
PubMed 29246575
DOI 10.1016/j.scr.2017.07.027

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