Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient
Skin fibroblasts and tumor fibroblasts were extracted from a 64-year old male patient clinically diagnosed with laryngeal carcinoma. Control and tumor specific induced pluripotent stem cells were reprogrammed with 5 reprogramming factors, Klf-4, c-Myc, Oct-4, Sox-2, and Lin-28, using the messenger RNA reprogramming system. The transgene-free iPSC lines showed pluripotency, confirmed by immunofluorescence staining. The iPSC lines also showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This in vitro cellular model can be used to study the oncogenesis and pathogenesis of laryngeal carcinoma. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Zhang Y, Zhu J, Dai Y, Wang L, Liu R|
|Journal||Stem cell research|
|Publication Date||2017 Dec;25:283-285|