An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene


Induced pluripotent stem cell (iPSC) line (SDQLCHi012-A) was generated from peripheral blood mononuclear cells of an 11-month-old male who was diagnosed as inflammatory bowel disease-28 caused by compound heterozygote for IL10RA mutations (c.188 + 1G > A and c.301C > T). Non-integrating episomal vectors coding OCT4, SOX2, KLF4, BCL-XL and MYC were used for reprogramming. The established iPSC line contained the same mutations identified in the patient, showed a normal karyotype, differentiation potential in vitro and expressed pluripotency markers. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Ma Y, Zhang H, Zhang S, Dong R, Yang X, Li Y, Guan J, Gai Z, Liu Y
Journal Stem cell research
Publication Date 2019 Dec;41:101577
PubMed 31648101
DOI 10.1016/j.scr.2019.101577

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