Isolation of single-base genome-edited human iPS cells without antibiotic selection


Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.

Authors Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR
Journal Nature methods
Publication Date 2014 Mar;11(3):291-3
PubMed 24509632
PubMed Central PMC4063274
DOI 10.1038/nmeth.2840

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