Isolation of single-base genome-edited human iPS cells without antibiotic selection
Summary
Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.
| Authors | Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR |
|---|---|
| Journal | Nature methods |
| Publication Date | 2014 Mar;11(3):291-3 |
| PubMed | 24509632 |
| PubMed Central | PMC4063274 |
| DOI | 10.1038/nmeth.2840 |