HS422
KIe029-A
General#
Cell Line |
|
| hPSCreg Name | KIe029-A |
| Alternative name(s) |
HS422
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 11th April 2019 |
| User feedback | |
Provider |
|
| Generator |
Karolinska Institutet (KI)
Contact:
Stem Cell Laboratory |
| Derivation country | Sweden |
External Databases |
|
| Cellosaurus | CVCL_C216 |
General Information |
|
| Publications |
|
| Is the cell line available in principle? |
Yes |
Donor Information#
General Donor Information |
|
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Regional ethical committee |
hESC Derivation#
| Date of derivation | 2005-11-07 |
| Embryo stage | Blastula with ICM and Trophoblast |
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
B
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Mechanical |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions#
| Feeder cells |
hFF Cellfinder Ont Id: CELDA_000001419 |
|||||||||
| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.