HS422
KIe029-A
General#
Cell Line |
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hPSCreg Name | KIe029-A |
Alternative name(s) |
HS422
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 11th April 2019 |
User feedback | |
Provider |
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Generator |
Karolinska Institutet (KI)
Contact:
Stem Cell Laboratory |
Derivation country | Sweden |
External Databases |
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Cellosaurus | CVCL_C216 |
General Information |
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Publications |
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* Is the cell line readily obtainable for third parties? |
Yes |
Donor Information#
General Donor Information |
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Phenotype and Disease related information (Donor) |
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Diseases |
Ethics#
Alternatives to consent are available? | Yes |
Alternatives to consent |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Regional ethical committee |
hESC Derivation#
Date of derivation | 2005-11-07 |
Embryo stage | Blastula with ICM and Trophoblast |
PGD Embryo? |
No |
Expansion status |
3![]() |
ICM morphology |
B![]() |
Trophectoderm morphology |
b![]() |
ZP removal technique | Mechanical |
Cell isolation | Mechanical |
Cell seeding | Isolated ICM |
Culture Conditions#
Feeder cells |
hFF Cellfinder Ont Id: CELDA_000001419 |
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O2 Concentration | 20 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-1 |
No |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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