Generation of induced pluripotent stem cell line, ZJUCHi002-A, from Charcot-Marie-Tooth disease type 2A (CMT2A) patient with a mutation of c.752C>T in MFN2

Summary

The human induced pluripotent stem cell (iPSC) line ZJUCHi002-A was established from renal epithelial cells present in urine (urinary cells) collected from an 8-year-old Charcot-Marie-Tooth disease type 2A (CMT2A) patient carrying point mutation in MFN2 (c.752C > T). Urinary cells were reprogrammed by retrovirus vectors containing reprogramming factors: OCT4, SOX2, KLF4 and c-MYC. The pluripotency, capacity of differentiation into 3 germ layers, silence of reprogramming factors and normal karyotype were all confirmed in this study. Copyright © 2019. Published by Elsevier B.V.

Authors Xu J, Fu Y, Xia W, He J, Zou Y, Ruan W, Lou Q, Li Y, Pan J, Li H, Chen W
Journal Stem cell research
Publication Date 2019 Feb 20;36:101411
PubMed 30807887
DOI 10.1016/j.scr.2019.101411

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