Generation of induced pluripotent stem cell line, ZJUCHi002-A, from Charcot-Marie-Tooth disease type 2A (CMT2A) patient with a mutation of c.752C>T in MFN2


The human induced pluripotent stem cell (iPSC) line ZJUCHi002-A was established from renal epithelial cells present in urine (urinary cells) collected from an 8-year-old Charcot-Marie-Tooth disease type 2A (CMT2A) patient carrying point mutation in MFN2 (c.752C > T). Urinary cells were reprogrammed by retrovirus vectors containing reprogramming factors: OCT4, SOX2, KLF4 and c-MYC. The pluripotency, capacity of differentiation into 3 germ layers, silence of reprogramming factors and normal karyotype were all confirmed in this study. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Xu J, Fu Y, Xia W, He J, Zou Y, Ruan W, Lou Q, Li Y, Pan J, Li H, Chen W
Journal Stem cell research
Publication Date 2019 Apr;36:101411
PubMed 30807887
DOI 10.1016/j.scr.2019.101411

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