Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats
Summary
We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Khurana P, Kolundzic N, Rogar M, Hobbs C, Wong XFCC, Common JEA, Ilic D, Liovic M |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 May;45:101827 |
| PubMed | 32361315 |
| DOI | 10.1016/j.scr.2020.101827 |