Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor
Summary
Using the integration-free episomal vector containing the reprogramming components OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28, and EBNA-1, hematopoietic stem cells obtained from a healthy 33-year-old man were effectively reprogrammed and turned into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were grown without the use of feeders. They exhibited a normal karyotype, displayed pluripotency markers, and differentiated into cells from the three germ layers. This DMSCi002-A line may serve as a control for investigating disease mechanisms. Copyright © 2025. Published by Elsevier B.V.
| Authors | Bumroongthai K, Yodtup C, Jantapalaboon D, Phairoh P, Suparak S, Dhepakson P, Tubsuwan A, Wongkidakarn S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2025 Jan 5;83:103655 |
| PubMed | 39778439 |
| DOI | 10.1016/j.scr.2025.103655 |