Generation of a human induced pluripotent stem cell line PUMCi001-A from a patient with Krabbe disease


We have generated PUMCi001-A, an induced pluripotent stem cells (iPSC) line from dermal fibroblasts of a 13-year-old male Krabbe disease patient with two hemizygous (461C > A and 1244G > A) mutations in Galactocerebrosidase (GALC) gene using a Sendai viral delivery of OCT4, SOX2, KLF4, and c-MYC. The PUMCi001-A iPSC line carried the GALC mutations, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited a normal karyotype and differentiation capacity into three germ layers. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wang W, Lv YF, Zhang YJ, Dong WJ, Zhang Y
Journal Stem cell research
Publication Date 2020 Oct;48:101937
PubMed 32763824
DOI 10.1016/j.scr.2020.101937

Research Projects

Cell Lines