HAD-C 104 cGMP-grade, xeno-free Single-cell, Feeder-free Master Cell Bank


Cell Line

hPSCreg name HADe011-A
Cite as:
Alternative name(s)
HAD-C 104 cGMP-grade, xeno-free Single-cell, Feeder-free Master Cell Bank
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 14th April 2022
Notes This cell bank is cGMP-grade, xeno-free, single-cell, and feeder-free; originally derived on umbilical-cord fibroblast feeders then expanded under feeder-free conditions.
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Generator Hadassah University Hospital (HAD)
Owner Hadassah University Hospital (HAD)
Derivation country Israel

External Databases

BioSamples SAMEA6583350
Cellosaurus CVCL_WW10
Wikidata Q93937763

General Information

* Is the cell line readily obtainable for third parties?
Research use: not allowed
Clinical use: allowed
Commercial use: allowed
Additional restrictions:

Contact us regarding matching research-grade cell line. Research use only generally not permitted unless goal is for clinical transplantation. Ethical restrictions apply.

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history Healthy donors
Is the medical history available upon request? Brief summary of donors available upon request

Karyotyping (Donor)

Has the donor karyotype been analysed?

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?

External Databases (Donor)

BioSamples SAMEA6583557


Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-20-0412
Is there other documentation provided to the donor for consenting purposes? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Hadassah IRB Committee
Approval number 33-26.07.02
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Hadassah ESCRO Committee
Approval number N/A

hESC Derivation

Date of derivation 2008-12-02
Supernumerary embryos from IVF treatment?
Separation of research and IVF treatment?
PGD Embryo?
Derived under xeno-free conditions?
Derivation under GMP?
Available as clinical grade?

Culture Conditions

Medium Other medium:
Base medium:
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
POU5F1 (OCT-4)
TRA 1-60
TRA 1-81
hESC Doubling Time (Potency)
Differentiation Potency
Columnar/cuboidal Epithelial Cell
Ont Id: CL_0000075
In vivo teratoma
Alpha Fetoprotein
Ont Id: Orphanet_173230
In vitro directed differentiation
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Actin, Aortic Smooth Muscle
Ont Id: NCIT_C103972
In vitro directed differentiation
Tubulin Beta 3 Class III
Ont Id: Orphanet_226013
In vivo teratoma
In vitro directed differentiation
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Normal Male Karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)