XFiPS-F44-3F-2
ESi032-A
General#
Cell Line |
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hPSCreg Name | ESi032-A |
Alternative name(s) |
XFiPS-F44-3F-2
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator | Spanish Stem Cell Bank (ES) |
Owner | Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB) |
Distributors | |
Derivation country | Spain |
External Databases |
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Cellosaurus | CVCL_C107 |
General Information |
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Publications | |
Projects | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unkown
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Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
foreskin fibroblastFibroblast from foreskin.
Synonyms
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Source cell origin |
prepuce of penisA retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
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Age of donor (at collection) | 1-4 |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Yes |
Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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Selection criteria for clones | morphological |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Gelatin | |||||||||
Feeder cells |
Human foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419 |
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O2 Concentration | 20 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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SOX2 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
ASA |
Yes |
SMA |
Yes |
GATA4 |
Yes |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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