Generation of two induced pluripotent stem cell lines from a patient with recessive inherited retinal disease caused by compound heterozygous mutations in SNRNP200
Summary
The human induced pluripotent stem cell (iPSC) lines LEIi015-A and LEIi015-B were derived from a patient with inherited retinal disease caused by compound heterozygous mutations in the SNRNP200 gene (c.[1792C>T];[3341T>C]). Dermal fibroblasts were transfected with episomal plasmids carrying transgenes encoding OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for P53. The clonal iPSC lines LEIi015-A and LEIi015-B expressed iPSC markers, were free from genomic alterations and demonstrated trilineage differentiation potential. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Zhang D, McLenachan S, Chen SC, Zaw K, Alziyadat Y, Zhang X, Lamey TM, Thompson JA, McLaren TL, Mellough C, De Roach JN, Chen FK |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Mar;51:102154 |
| PubMed | 33429167 |
| DOI | 10.1016/j.scr.2020.102154 |